Infect Control Hosp Epidemiol 2005 26:515–9. Tetrasodium EDTA as a novel central venous catheter lock solution against biofilm. Percival SL, Kite P, Eastwood K, Murga R, Carr J, Arduino MJ, et al. Changes in the cell wall glycoprotein composition of Candida albicans associated to the inhibition of germ tube formation by EDTA. Inhibition of Candida albicans biofilm formation by farnesol, a quorum-sensing molecule. Ramage G, Saville SP, Wickes BL, Lopez-Ribot JL. Function of Candida albicans adhesin Hwp1 in biofilm formation. Nobile CJ, Nett JE, Andes DR, Mitchell AP. albicans transcription factor Bcr1p: a regulator of cell-surface genes and biofilm formation. The filamentation pathway controlled by the Efg1 regulator protein is required for normal biofilm formation and development in Candida albicans. Ramage G, VandeWalle K, Lopez-Ribot JL, Wickes BL. albicans biofilm formation in vitro and in vivo. Critical role of Bcr1-dependent adhesins in C. Nobile CJ, Andes DR, Nett JE, Smith FJ, Yue F, Phan QT, et al. Role of dimorphism in the development of Candida albicans biofilms. Genetics and genomics of Candida albicans biofilm formation. Candida albicans biofilm development, modeling a host-pathogen interaction. Candida albicans biofilms: more than filamentation. In vitro activity of eugenol against Candida albicans biofilms. Candida Albicans: a molecular revolution built on lessons from budding yeast. Biofilm formation: a clinically relevant microbiological process. Bacterial biofilms: a common cause of persistent infections. Eukaryot Cell 2005 4:633–8.Ĭosterton JW, Stewart PS, Greenberg EP. Ramage G, Saville SP, Thomas DP, Lopez-Ribot JL. The use of new probes and stains for improved assessment of cell viability and extracellular polymeric substances in Candida albicans biofilms. Jin Y, Zhang T, Samaranayake YH, Fang HH, Yip HK, Samaranayake LP. Candida biofilms on implanted biomaterials: a clinically significant problem. Effects of dietary sugars and, saliva and serum on Candida bioflim formation on acrylic surfaces. Nikawa H, Nishimura H, Hamada T, Kumagai H, Samaranayake LP. Eur J Clin Microbiol Infect Dis 2002 21:767–74. Candidemia at a tertiary-care hospital: epidemiology, treatment, clinical outcome and risk factors for death. Viudes A, Peman J, Canton E, Ubeda P, Lopez-Ribot JL, Gobernado M. National Nosocomial Infections Surveillance System. Secular trends in the epidemiology of nosocomial fungal infections in the United States, 1980–1990. This compound may serve a non-toxic means of preventing biofilm formation on infections with a C. albicans biofilm etiology.īeck-Sague C, Jarvis WR. These results indicate that EDTA inhibits C. albicans biofilm formation are most likely through its inhibitory effect on filamentation and indicates the potential therapeutic effects of EDTA. The HWP1 gene expression was reduced in EDTA-treated planktonic and biofilm samples. However, preformed biofilms were minimally affected by EDTA (maximum of 31% reduction at 250 mM). Microscopic analysis and colorimetric readings revealed that filamentation and biofilm formation were inhibited by EDTA in a concentration dependant manner. Northern blot analysis of the hyphal wall protein ( HWP1) expression was also monitored in planktonic and biofilm cells treated with EDTA. The extent and characteristics of biofilm formation were then microscopically assessed and with a semi-quantitative colorimetric technique based on the use of an XTT-reduction assay. All plates were then incubated at 37☌ for an additional 24 h to allow for biofilm formation. EDTA was also added to the standardized suspension prior to adding to the microtiter plate and to a preformed 24 h biofilm. Cells were allowed to adhere for 1, 2, and 4 h at 37☌, washed in PBS, and then treated with different concentrations of EDTA (0, 2.5, 25, and 250 mM). Candida albicans biofilms were formed in 96-well microtitre plates. In this study we investigated a means of inhibiting biofilm formation using EDTA (Ethylenediaminetetra-acetic acid), a divalent cation chelating agent, which has been shown to affect C. albicans filamentation. Candida albicans can readily form biofilms on both inanimate and biological surfaces.
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